ZFIN Image: Yoo et al., 2010, Fig. 6

Image

Figure Caption

Fig. 6 PI(3)K Regulates Anteroposterior Polarity of F-Actin Dynamics in a Pathway That Is Separable from Rac-Mediated Protrusion

(A) In control, photoactivation of Rac at the leading edge induces protrusion at the leading edge with GFP-UtrCH (stable F-actin) localized at the tail (Movie S13A).

(B) Photoactivation of Rac with PI(3)K inhibition induces protrusion with GFP-UtrCH (stable F-actin) localized at the leading edge, indicating reverse polarity of F-actin dynamics (Movie S13B).

(C) Protrusion induced by photoactivation of Rac induces PHAKT-EGFP accumulation at the leading edge, suggesting a positive feedback from Rac to PI(3,4,5)P₃-PI(3,4)P₂ gradient (Movie S14).

(D) A schematic representation of two-tiered PI(3)K-mediated regulation of cell motility: PI(3)K promotes Rac-mediated actin polymerization at the leading edge while generating anteroposterior polarity of F-actin dynamics. Images are representative of more than five time-lapse movies from experiments repeated on at least two separate dates.

Scale bars = 10 μm.

Acknowledgments

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Reprinted from Developmental Cell, 18(2), Yoo, S.K., Deng, Q., Cavnar, P.J., Wu, Y.I., Hahn, K.M., and Huttenlocher, A., Differential Regulation of Protrusion and Polarity by PI(3)K during Neutrophil Motility in Live Zebrafish, 226-236, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell