Fig. 8

Fig. 8 Uhrf1 and Dnmt1 are required lens-autonomously for normal lens development. Shield-stage transplants were performed as described in Fig. S6. (A–F) Representative transplants between wild-type embryos and uhrf1 mutants and (G–L) wild-type embryos and dnmt1 mutants. All images are from 5 dpf embryos and each pair of whole-mount and histology images is derived from the same embryo and eye. For consistency, all data presented are taken from embryos with medium (30–70%) contribution of cells in the mosaic host lens. (A,D,G,J) whole-mount and histology images of eyes formed from donor embryos, (B,E,H,K) whole-mount and histology images of eyes formed from the non-mosaic side of host embryos and (C,F,I,L) whole-mount and histology images of eyes formed from the mosaic lens. Mutant to wild-type (A–C,G–I) transplants and wild-type to mutant transplants (D–F,J–L) both yielded normal lens formation in the mosaic host lens indicating that Uhrf1 and Dnmt1 function is required lens autonomously for lens development. Scale bars are 80 μm.