ZFIN Image: Wang et al., 2011, Fig. S4

Image

Figure Caption

Fig. S4 Cre-responder line capable of reporting on exocrine fate mapping in 5 dpf larvae. Confocal images of single optic sections of micro-dissected pancreata from β-actin:eGFP-F2A-cre^ERT2; β-actin:loxP-stop-loxP-hmgb1-mCherry 5dpf larvae. (A,B) Following 4OHT addition at 56 hpf, cells within the pancreas parenchyma are labeled with the lineage tracer Hmgb1-mCherry. (A) Counterstaining for nuclei in blue indicates number of cells in field. Red nuclei indicate lineage-traced cells. (B) Merged red and bright-field images show that these labeled or lineage-traced cells include zymogen granule filled acinar cells of the exocrine pancreas. (C,D) Without 4OHT treatment, no labeling is detected.

Acknowledgments

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