Fig. S3

Fig. S3 Cell death assay of rumba^hkz1 and samba^hkz2 mutant embryos. (A-X) TUNEL and Cd41-GFP double staining of 3 dpf, 4 dpf and 5 dpf rumba^hkz1 mutants (E-H,M-P,U-X) and wild-type siblings in the CHT (A-D,I-L,Q-T). (A′-X′) TUNEL and Cd41-GFP double staining of 3 dpf, 4 dpf and 5 dpf samba^hkz2 mutants (E′-H′,M′-P′,U′-X′) and siblings in the CHT (A′-D′,I′-L′,Q′-T′). Arrows indicate GFP and TUNEL double positive cells. Arrowheads indicate GFP negative but TUNEL positive cells. (Y) Quantification of TUNEL and Cd41-GFP double-positive cells in the CHT of rumba^hkz1 mutants and siblings. (Y′) Quantification of TUNEL and Cd41-GFP double-positive cells in the CHT of samba^hkz2 mutants and siblings. Error bars indicate s.d.