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Fig. S2 Hypoxia delays the timing of brain and somite morphogenesis but does not change patterning. (A) Whole-mount in situ hybridization of pax2a of embryos at 32 hpf in normoxia (stage-matched control), at 48 hpf in normoxia, and at 48 hpf in hypoxia. mbh, midbrain-hindbrain boundary; os, optic stalk. Scale bar: 200 μm. 12-hpf embryos were transferred to hypoxic or normoxic water, raised to the indicated stages, and subjected to whole-mount in situ hybridization. (B) 12-hpf embryos were transferred to hypoxic or normoxic water, raised to 24 hpf, and subjected to whole-mount in situ hybridization using the indicated markers. No pattern differences were found.