ZFIN Image: Hong et al., 2011, Fig. S1

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Figure Caption

Fig. S1 Recapitulation and rescue of epiboly defects in Mxtx2 morphants. (A-D) Embryos were injected at the 1-cell stage with (A) 8 ng Ctrl MO, (B) 1 ng Mxtx2 MO, (C) 2 pg mxtx2 mRNA + 1 ng Mxtx2 MO or (D) 2 pg mxtx2 mRNA alone, and documented by time-lapse in their chorions, as previously described (Pei et al., 2007; Schroter et al., 2008). Images show embryo morphologies at the time that control embryos reached 100% epiboly. (E) A total of 159 embryos that were time-lapsed or incubated in parallel were scored, revealing a highly significant degree of rescue. ***, P<0.0001; χ² test.

Pei, W., Noushmehr, H., Costa, J., Ouspenskaia, M. V., Elkahloun, A. G. and Feldman, B. (2007). An early requirement for maternal FoxH1 during zebrafish gastrulation. Dev. Biol. 310, 10-22.

Schroter, C., Herrgen, L., Cardona, A., Brouhard, G. J., Feldman, B. and Oates, A. C. (2008). Dynamics of zebrafish somitogenesis. Dev. Dyn. 237, 545-553.

Acknowledgments

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