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Fig. S4 Chemically induced inflammation assay (ChIn) using Sudan Black. (a and b) Bright-field images of untreated (a) and 10 μM CuSO4-treated (b) 56-hpf casper larvae stained with Sudan Black to reveal leukocytes. Note the congregation of labeled cells at the posterior lateral line neuromasts (arrows). (c) Quantification of leukocyte migration (detected by Sudan Black staining) to the lateral line in untreated and metal-exposed larvae. The result is equivalent to that obtained with BACmpx::GFP or lysC::DsRED2 transgenic larvae.
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