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Figure Caption
Fig. 3 Cfl1 MOs efficiently block the translation of GFP fusion constructs containing respective MO binding site.
Embryos at 1-cell stage were injected with 330 pg pCS2+ XLT fused with a fragment of cfl1 gene sequence from -22 to +195 or -52 to +51 in the absence or presence of 7.5 ng cfl1 tMO1 or tMO2, respectively. The expression of GFP was examined at 10 hpf and photographed under epifluorescent microscopy. The representative photos for embryos treated without cfl1 MO (A) and with tMO1 (B) are shown and the percentages of injected embryos expressing GFP are shown in average ± standard deviation (C).
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