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Fig. S1 Cre-mediated recombination of the (hsp70l:mCherry-T2A-CreERT2)#12 allele in the red-to-green reporter line in the absence and presence of heat and TAM. A: Scheme of the transgene combination of the embryos depicted in B–E. B: In the absence of heat and TAM, the combination of the Tg(hsp70l:mCherry-T2A-CreERT2)#12 allele with the red-to-green reporter line results in strong DsRed2 but no EGFP expression in double transgenic embryos. C: Application of TAM leads to mosaic EGFP expression in double transgenic embryos at permissive temperatures. D: In the absence of TAM, mosaic EGFP expression can be detected after heat treatment. E: Application of TAM and heat results in strong ubiquitous EGFP expression in double transgenic embryos. B–E: Lateral views of live 24-hpf embryos.