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Fig. S8 Synergy between vHnf1 and MafB for the activation of endogenous Krox20 in chick. Chick embryos were analysed by in situ hybridisation for Krox20 after electroporation with expression vectors for (A) vHnf1, (B) MafB or (C) both. Hindbrains were flat-mounted with anterior to the top. With vHnf1, limited ectopic Krox20 expression occurs specifically in r4. With MafB, limited ectopic activation of Krox20 is observed in the posterior hindbrain. With both expression vectors together, ectopic expression occurs more frequently and throughout the entire hindbrain.
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