ZFIN Image: Labalette et al., 2011, Fig. S3

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Figure Caption

Fig. S3 Hindbrain patterning defects resulting from Spry4mo injection are persistent and can be rescued by injection of spry4 mRNA. (A-C) Embryos were injected with either control morpholino (Ctrlmo) and gfp mRNA (A), Spry4mo and gfp mRNA (B), or Spry4mo and a morpholino-resistant form of spry4 mRNA (C), collected at the 20-somite stage and subjected to in situ hybridisation for krox20 and her5, a marker of the MHB. (D) Quantitative evaluation of rhombomere areas, carried out as described in Fig. 2. ns, not significant, P>0.05; *, not significant after Bonferroni corrections considering 12 comparisons, P>0.004; **, P<0.004; ***, P<0.0001; t-test. Errors bars indicate s.e.m.

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