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Fig. S2 Keratinocyte damage causes immediate axon fragmentation following axotomy. Two-photon image stack projections of four different trigeminal axons (A-D) and neighboring keratinocytes expressing GFP. (A-D) Skin cells (marked by pink dotted lines) that overlap with axons before axotomy and ablation. Two-photon laser ablation of skin cells was performed concurrently with axotomy of the adjacent axon. (A′-D′) Immediately after ablation/axotomy. Pink dotted lines indicate damaged/dead keratinocytes, and yellow arrowheads indicate axons that instantly fragmented (n=5). Scale bars: 50 μm.
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