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Fig. S4

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ZDB-IMAGE-101129-3
Source
Figures for Chung et al., 2010
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Figure Caption

Fig. S4 Verification of the alk8 splice-blocking morpholino by comparing alk8 mutants with alk8 morphants, and RT-PCR. (A–D) Morphology of the tail at 30 hpf. (A and C) Wild-type and alk8 heterozygote embryos display normal tail development. (B) alk8 mutants display a dorsalized phenotype resulting in a lack of the ventral tail fin. (D) All embryos injected with the alk8 morpholino exhibit ventral tail fin defects (75% of the embryos lacked the whole ventral fin and 25% had a partial reduction of the fin). (E) RT-PCR showing reduced amount of spliced alk8 mRNA in embryos injected with alk8 morpholino at 30, 56, and 72 hpf. Twenty embryos from each condition and stage were used for cDNA synthesis. The alk8 morpholino blocks the splice donor site of the second coding exon, leading to a decreased PCR product because the primers used anneal to the second (52-ctgctagtcatgtggtagaatgctg-32) and fifth (52-cacttcaccgtaccgtcctt-32) coding exons. Equal amounts of cDNA template is indicated by amplification of elongation factor 1-α, using the primers (52-tcaccctgggagtgaaacagc-32) and (52- acttgcaggcgatgtgagcag-32).

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