ZFIN Image: Sinha et al., 2010, Fig. 2

Image

Figure Caption

Fig. 2 Cre-mediated recombination in the green-to-red reporter line. In Tg (ef1a: loxP-GFP-loxP-dsRed2) embryos injected with 3 pg of Cre mRNA at one-cell stage (A), dsRed fluorescence is ubiquitously observed at 36 hpf. Embryos were injected with 3 pg of CreER^T2 mRNA, further incubated with embryo medium (B) or 1 μM cyclofen-OH (Ind) (C), and observed at 2 dpf. Variation of the recombination efficiency with the amount of injected CreER^T2 mRNA (in pg) and inducer concentration: 0 μM (blue triangle) or 3 μM (red square). The percentage of embryos exhibiting dsRed fluorescence was scored by epifluorescence microscopy at 2 dpf (D). The error bars represent statistical errors estimated as √p(1-p)/n. Scale bars: 100 μm. dpf, days postfertilization.

Acknowledgments

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