Fig. S5

Fig. S5 Combined treatment with Dox and Dex or with Dox and Tebufenozide is not toxic for adult zebrafish. (A) Average regenerate length at 8 d postamputation (dpa) of fish intraperitoneally injected with the indicated doses of drugs at 1 dpa, 3 dpa, and 6 dpa. Zero indicates EtOH vehicle only. Regenerate length is not significantly different (Student t test). Error bars indicate SEM. n = 6/group. (B and C) Representative regenerating fins at 7 dpa of fish housed in water containing vehicle or 25 μg/mL Dox plus 10 μM Tebufenozide. Note robust regeneration in both. Amputation plane is indicated by arrowheads. (D) Average regenerate length measured at 7 dpa of fish incubated with the indicated doses of drugs from 0.5 dpa. Zero indicates vehicle only. Regenerate length is not significantly different between vehicle control and drug-treated fins (Student t test). Error bars are SEM. n = 6/Group. (E) Average regenerate length at 8 dpa of fish intraperitoneally injected with the indicated doses of drugs at 1 dpa, 3 dpa, and 6 dpa. Zero indicates vehicle only. Regenerate length is not significantly different (Student t test). Error bars indicate SEM. n = 6/Group.