ZFIN Image: Lugo-Villarino et al., 2010, Fig. 4

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Figure Caption

Fig. 4

Further enrichment of zebrafish DCs for functional analyses. (A) Single-cell suspensions were prepared from mpx:eGFP transgenic WKM and labeled with PNA. Myelomonocytes (green gate) were divided into mpx^- (black box) and mpx⁺ (blue box). These populations were further subdivided into mpx^- PNA^- (gray box), mpx^- PNA⁺ (red box), and mpx⁺ PNA⁺ (blue box) fractions. (B) After 4 h of culture, mpx^- PNA^- (gray), mpx^- PNA⁺ (red), and mpx⁺ PNA⁺ (blue) fractions were collected and stained with MGG to assess cell morphology. Differentials are presented as mean � SD, n = 6. (C) mpx^- PNA⁺ (red bar), mpx⁺ PNA⁺ (blue bar), and mpx^- PNA^- (black bar) myelomonocyte fractions were cultured for 16 h with and without LPS. The abundance of il-12p40, csf1r, and iclp1 transcripts were measured by qPCR. Expression is presented as fold induction over nonstimulated controls. Asterisks denote populations with no detectable expression of the transcript. Bars represent mean, n = 3. Error bars represent SD.

Acknowledgments

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