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Fig. S3 Comparative mRNA in situ hybridization analysis to determine the onset of expression in rhombomeres 3 and 5 of (A–C) egr2b in WT embryos, (D–F) kalTA4 and (G–I) gfp in offspring of rh3/5:KalTA4 X 4xKGFP cross. (J–L) mRNA in situ hybridization analysis of downregulation of expression of kalTA4 and (M–O) gfp in rhombomeres 3 and 5 of rh3/5:KalTA4 X 4xKGFP embryos. Arrows indicate expression domains of the respective genes.
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