ZFIN Image: Koide et al., 2009, Fig. S3

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Figure Caption

Fig. S3 Differential GFP expression in distinct types of OSNs in 2 transgenic fish lines, SAGFF27A (A–D) and OMP:Gal4FF (E–H). Adult OE sections were triple-labeled with antibodies against GFP (A and E; green in D and H), calretinin (B and F; red in D and H), and Gα_olf/s (C and G; blue in D and H). In the SAGFF27A line, GFP is expressed in short dendrite-bearing and calretinin-positive OSNs in the apical layer of the OE (A, B, and D), suggesting that Gal4FF/GFP-expressing cells are the microvillous OSNs. In contrast, in the OMP:Gal4FF fish, GFP is observed in long dendrite-bearing OSNs, the cell bodies of which are located in the basal layer of the OE (E, G, and H). Arrows indicate the dendritic knobs that are positive for both GFP and Gα_olf/s on the epithelial surface. These results indicate that Gal4FF/GFP-expressing cells in the OMP:Gal4FF fish are the ciliated OSNs. (Scale bar: 10 μm.)

Acknowledgments

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