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Fig. S4 Invasion, dissemination and metastasis of LLC-VEGF tumors. (A and D) DiI-labeled LLC-vector and LLC-VEGF tumor cells were implanted in the perivitelline space and tumor cell invasion and dissemination were examined at day 6 post-injection. Arrows indicate primary tumors. Yellow arrowheads in (D) indicate pericardium edema. White arrowheads indicate disseminated tumor foci. (Scale bar, 500 μm.) (B and E) High-resolution micrographs of A and D, respectively to visualize single metastatic tumor cells. (Scale bar, 100μm.) (C and F) Representative 3-D micrographs of confocal images of tumors (red) and tumor vasculatures (green). (Scale bar,10 μm.) (G) Quantification of tumor volume (n = 28/group). (H) Quantification of numbers of disseminated tumor foci (n = 28/group). (I) Averages of maximal distances of metastatic foci (n = 28/group). (J) Quantification of tumor vessel density relative to tumor sizes (n = 7/group). Data are represented as mean ± SEM.