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Fig. S1 Ntl binds and regulates expression of genes involved in muscle specification and morphogenetic movements. (A) GRN showing Ntl binding and regulation of factors involved in specifying muscle cell fate. Solid lines indicate binding of target promoter and genetic regulation. Dashed line indicates target binding only or genetic regulation without proven target binding. (B–C′) In situ hybridization of ntl mutants compared to wild types at gastrula and bud stages shows downregulation of foxd3 (arrows). (D–F) Ntl binding in genomic regions around foxd3, myod, and msgn1. Plots show ChIP-enrichment ratios for microarray probes in the genomic regions shown. Chromosomal position, TSS, intron-exon structure, putative upstream T-binding sites with constrained character (green lines; see text), and conserved CRM (red bar; see text) are shown below the graphs. (G) GRN showing Ntl binding and regulation of genes involved in morphogenetic movements. (I–J′) In situ hybridization shows downregulation of blf in ntl mutant compared to wild-type embryos at 70% epiboly and bud stage. (H–M) Ntl binding in genomic regions around blf, snai1a, wnt11, and cx43,3. a, anterior; d, dorsal; v, ventral