|
Fig. 3
Ntl binds and regulates the expression of genes involved in posterior identity. (A) Diagrams to show fgf and wnt signaling pathways. Components outlined in black were identified as Ntl targets by ChIP-chip. (B–F) Ntl binding in genomic regions around wnt8, fgf24, vent, vox, sp5l, and aldh1a2. Plots show ChIP-enrichment ratio for microarray probes in the genomic regions. Chromosomal position, TSS, intron-exon structure, putative upstream TBSs with constrained character (green lines; see text), and conserved CRMs (red bar; see text) are shown below the graphs. (G–N2) In situ hybridization of ntl mutants compared to wild types for vent, vox, sp5l, and aldh1a2 (posterior views, ventral down). Expression of vent, vox, and sp5l shows downregulation at bud stages (arrows). In addition expression of sp5l is absent from the dorsal forerunner cells in ntl mutant embryos (asterisk; DFCs outlined by dashed line). aldh1a2 expression in the ventral margin is downregulated at gastrula stages.