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Fig. 7 Simultaneous knockdown of katna1 and spast enhances phenotypic severity. Immunostaining with znp-1 demonstrates that embryos co-injected with 0.3 pmol of spg4atg1 and 0.9 pmol of katna1aug1 morpholinos showed far more severe defects in spinal motor axon outgrowth (D) than embryos injected with either 0.3 pmol of spg4atg1 (B) or 0.9 pmol of katna1aug1 (C) alone. (A) Control embryo injected with 1.2 pmol of a control morpholino. (E) Quantification of mean spinal motor axon length in morpholino-injected embryos. Statistical significance was determined using ANOVA with Bonferroni’s multiple comparison test: ns, not significant; ** P<0.01; ***P<0.001. Scale bars: 50 μm.