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Fig. S9 Expression of three marker genes at 36 hpf in the heart of normal and klf2a morphants. (A and B) cmlc2 expression is essentially normal in the klf2a morphants, showing that chamber specification occurs independently of klf2a. (C and D) bmp4 mRNA distribution at 36 hpf showing that expression is normal in the MO-treated embryo in the AV node region at that stage. (E and F) notch1b expression decreases in the AV boundary of the klf2a morphants at 36 hpf (n = 24, 63%; compare expression at tip of arrows). Arrows point to the AV boundary in all panels.
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