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Fig. 10
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Fig. 10 Tsc2 knockdown induces precocious expression of intestinal markers. A: RT-PCR demonstrating knockdown of tsc2 in spl MO-injected embryos. B: Western blot of extract from tsc2 spl morphants at 53 hpf for P-RPS6ser240/244, showing upregulation of TORC1 signaling. C: qRT-PCR of tsc2 morphant RNA for markers of intestinal differentiation showing upregulation of ifabp and iap expression at 48 and 72 hpf, and slc10a2 at 96 hpf. Samples were normalized to ef1α and significance was determined using Student′s two-tail, unpaired t-test.
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