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Fig. 9 Knockdown of lkb1 induces precocious expression of intestinal markers. A: In situ hybridization to intestinal fatty acid binding protein (ifabp) at 48 hpf. Arrow indicates the gut tube, showing increased expression in the lkb1 morphant. Original magnification, 40x. B: Relative mRNA expression of intestinal markers ifabp and iap at 48 and 72 hpf, and slc10a2 at 96 hpf in uninjected, spl, ATG, 5 mm, and 25N determined by qRT-PCR. Samples were run in triplicate and normalized to ef1α to control for total RNA. Significance was determined using Student′s two-tail, unpaired t-test. C: Rhodamine-conjugated wheat germ agglutinin (WGA) staining for goblet cells in the uninjected and lkb1 spl morphant at 96 and 121 hpf. Very few goblet cells are visible in the control embryo, whereas the morphant displays a much brighter signal. At 121 hpf, the fluorescent signals are qualitatively similar between control and morphant. White line encircles the hindgut. Original magnification: 40x.