|
Fig. S4 Alternate view of the tailbud and presomitic mesoderm in wnt8, chordin, and wnt8;chordin morphants. All images are lateral views, 5-6-somite stage embryos, anterior to left, dorsal up. Genotypes are indicated above each column. Brackets indicate the tailbud, defined as tissue posterior to presomitic mesoderm. A-D: tbx24 is expressed in presomitic mesoderm. Note the strong reduction in tailbud tissue observed in wnt8 morphants (B), significant expansion in chordin morphants (C), and approximately wild-type size observed in wnt8;chordin morphant (D). E-H: Lateral views of embryos stained for myf5 expression reveal a similar result. Note that myf5 expression is also in adaxial cells that flank the notochord into the tailbud, thus the apparent size of the tailbud in this view is smaller than that observed in tbx24-stained embryos. This is thus an optical effect, yet the result is not different between the two probes used.