ZFIN Image: Baker et al., 2010, Fig. 3

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Figure Caption

Fig. 3 Wnt8 is not required for BMP signaling activity. Images of anti-phosphorylated Smad1/5 staining. Genotypes are indicated above each row. Arrows indicate dorsal extent of visible labeling. A-D: Animal pole views of shield stage embryos, dorsal right. E-H: Lateral views, shield stage, dorsal right. I-L: Lateral views, -80% epiboly, anterior up, dorsal right. P-Smad1/5 is confined to the ventral embryo at shield stage (A,E), and expression is significantly reduced in wnt8 morphants (B,F). P-Smad1/5 expands dorsally (arrows) in chordin morphants (C,G) and in wnt8;chordin morphants (D,H). P-Smad1/5 can still be detected in a dorsally expanded domain of chordin (K) and wnt8;chordin (L) morphants in late gastrulation. Inset in L: vegetal view, dorsal right; arrows indicate dorsal extent of staining. Note absence of staining in wnt8 morphant at 80% epiboly (J).

Acknowledgments

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