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Fig. 6 ErbB2 is required cell-autonomously in cardiomyocytes for the initiation of cardiac trabeculation. (A,B) Chimeric ventricles resulting from transplanting wild-type (A) or erbb2 mutant (B) blastomeres from Tg(cmlc2:dsRed) donors into Tg(cmlc2:GFP) wild-type hosts. Wild-type donor cells were detected in both the compact and trabecular layers, whereas erbb2 mutant donor cells failed to incorporate into the trabeculae (arrows). (C) Chimeric ventricles resulting from transplanting wild-type blastomeres from Tg(cmlc2:GFP) donors into Tg(cmlc2:dsRED); erbb2 mutant hosts. Note that the trabeculae only contain wild-type cells. (D) Quantification of the distribution of the wild-type and mutant donor-derived cells in the compact versus trabecular layers in the chimeric ventricles, assessed by measuring the surface area of the donor-derived cells.