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Fig. S4 Migratory DFCs are not properly formed in αV morphants. In addition to cas (Fig. 4), sox17 and ntl expression in DFCs was also used to assess DFC location in integrin morphants at 80% E. Dorsal views of embryos were slightly tilted to visualize sox17 (A-E) and ntl (F-J) expression in DFCs. (K,L) Bar graphs showing scores from DFC phenotypes for each DFC marker. Phenotypic classification of DFCs were as follows: wild-type (WT), ovoid DFC marker expression domain; mutant, a linear DFC marker expression domain with occasional gaps; none, no visible DFC marker expression. Data are expressed as the number of embryos with DFC phenotypes divided by the total number of embryos used per experiment multiplied by 100 (%) ± s.e.m. Scale bars: 20 μm in A-E; 100 μm in F-J. See also Table S4 in the supplementary material.