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Fig. S5 Cell death does not persist in dmbx1 morphant embryos. Live embryos at 24 hpf (A, B, lateral view anterior to the left), 48 hpf (C, D, dorsal view anterior to the left) and 72 hpf (E, F, dorsal view anterior to the left; K, L, close up of retina lateral view dorsal to the top) were examined for the presence of apoptotic cells using AO. Arrows point to AO+ cells in the midbrain (bright spots). TUNEL+ cells (green) or Caspase3+ cells (red) on cryosectioned tissue of 72 hpf midbrains (G-J) and retina (M-P, arrow pointing to labelled cell) counter-stained with DAPI (blue). (MO, morpholino; mMO, mismatch morpholino; hpf, hours post-fertilization; AO, acridine orange.