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Figure Caption
Fig. 2 Expression of ptc1 in two Hh pathway mutants and in embryos in which shh RNA has been overexpressed. Dorsal views of 24 hpf whole-mount otic vesicle preparations showing ptc1 expression. Anterior towards the left, lateral towards the bottom. Scale bar: 50 μm. Note that ptc1 expression is much weaker in contf18b homozygotes (B) than in wild-type embryos (A; a sibling of the contf18b homozygote). ptc1 expression is undetectable in the smub641 otic vesicle (C) and is upregulated throughout the vesicle in embryos in which shh has been overexpressed by injection of 100ng/μl shh RNA (D).
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