Fig. 4

Fig. 4 Gene expression in con^tf18b and smu^b641 ears. Whole-mount in situ hybridisation; anterior towards the left. (A-C,J-O,Y-Aa) Dorsal views, medial towards the top. All other panels are lateral views, dorsal towards the top. Anterior otic expression domains of otx1 (A-C), wnt4 (D-F) and nkx5.1 (G-I), but not pax5 (J-L) and fgf8 (M-O), are duplicated at the posterior of smu and con otic vesicles. Arrowhead (A,B) shows axis of otx1 symmetry in smu ears. Arrow (A,C) shows axis of otx1 symmetry in con ears. nkx5.1 expression in the statoacoustic ganglion (g) is not duplicated at the posterior of the ear (G-I). Posterior expression domains of follistatin (arrowhead, P-R) are lost. Expression of dorsal (dlx3b, S-U), ventral (eya1, V-X) and medial (pax2a, Y-Aa) markers are not affected in con and smu. An ectopic expression domain of the crista marker msxc is present at the posterior of ∼31% con and 50% smu mutants (Ab-Ad). ot.v, otic vesicle; g, statoacoustic ganglion. Scale bars: 50 μm (shown in the left-hand panel of each set).