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Fig. S2 PCP signalling regulates the laterality of Nodal asymmetric gene expression in zebrafish. (A-D) Expression of the Nodal-related gene southpaw revealed by in situ hybridisation in 18-somite stage slb mutant embryos injected with 1.5 ng of pk1a-MO. (E) Horizontal bar chart showing the relative frequency of southpaw asymmetric gene expression (left, yellow; bilateral, light blue; right, red; absent, green) scored in different experimental conditions: control (wild-type untreated, n=85); slbwnt11 (silberblick) mutants, n=146; pk1a-MO 3 ng (wild-type injected with 3 ng of pk1a-MO at 1-cell stage, n=27); slb;pk1a-MO 1.5 ng (slb mutants injected with 1.5 ng of pk1a-MO at 1-cell stage, n=96). (F-H) Expression of the Nodal signalling component lefty2 revealed by in situ hybridisation in 22-somite stage slb mutant embryos injected with 1.5 ng of pk1a-MO. (I) Horizontal bar chart showing the relative frequency of lefty2 asymmetric gene expression (left, yellow; right, red; absent, green) scored in different experimental conditions: control (n=43); slb (n=60); pk1a-MO 3 ng (n=50); and slb;pk1a-MO 1.5 ng (n=41). Scale bars: 100 μm.