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Fig. 4 Knockdown of gpr177 mRNA expression. An antisense gpr177 MO was used to knock down gpr177 mRNA translation. All images are lateral views with anterior to the right. Morphants produced by the ATG-MO are in C, D, G, H, while morphants produced by the UTR-MO are in J, K. A: 24 hpf wild type embryo. B: 24 hpf, head of wild type embryo. C: 24-hpf morphant injected with 4 ng of ATG-MO. D: Head of 24-hpf morphant. E: 48-hpf wild type embryo. F: Head of 48-hpf embryo. G: 48-hpf embryo injected with 4 ng ATG-MO. H: Head of 48-hpf morphant. J: 48-hpf embryo injected with 6 ng of UTR-MO. K: Head of 48-hpf morphant. Arrows indicate otoliths. Arrowhead indicates protrusions of semicircular canals. MHB, midbrain-hindbrain boundary; OV, otic vesicle; CE, cardiac edema. L: Phenotypes of morphants injected with UTR-MO scored at 48 hpf. N = 19–21 at each dose. M: UTR-MO injected embryos (scored in L) were harvested at 48 hpf and GPR177 expression compared to that in wild type embryos. Arrowhead indicates position of zebrafish GPR177 (<43 kDa).