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Fig. S1 Knockdown expression of Plk1 results in delay in mitosis. Images represent the result from Fig. 2D. Embryos were injected with 0.25 pmol of control or plk1 ATG MO and pulse-labeled with BrdU at 24 hpf for 30 min, washed and incubated for the time points indicated. They were then fixed and co-immunostained with anti-BrdU and anti-pH3 antibodies. Cells double positive for BrdU and pH3 marks the cells in mitosis.
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Reprinted from Developmental Biology, 345(1), Jeong, K., Jeong, J.Y., Lee, H.O., Choi, E., and Lee, H., Inhibition of Plk1 induces mitotic infidelity and embryonic growth defects in developing zebrafish embryos, 34-48, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.