Fig. 6

Fig. 6 Treatment of 24 hpf embryos with BI 2536, the small molecule inhibitor specific for Plk1, leads to mitotic infidelity and prometaphase arrest. (A) Scheme of BI 2536 treatment in developing zebrafish embryos. Embryos at one-to four-cell stage were injected with indicated morpholino oligos and the drug was treated at 24 hpf. (B) Captured images of cells from the control embryos. The black arrow marks the timing of anaphase onset. The time at NEBD was set to 0. (C–G) Captured images of BI 2536-treated embryos after the injection of indicated morpholinos (supplemental movies 10-14). (H) Box plots of timing from NEBD to mitotic exit. More than 50 cells from at least five embryos each were analyzed. Bars within the boxes are the median values, determined by statistical analysis using SPSS software. Note that BI 2536 treatment in plk1 morphants exhibit more severe phenotype compared to others. (I) Immunofluorescence of the fixed embryos with anti-α-tubulin and -γ-tubulin with or without BI 2536 treatment. Note the monopolar spindles in BI 2536-treated embryos.