ZFIN Image: Jeong et al., 2010, Fig. 3

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Figure Caption

Fig. 3 Plk1 depletion leads to prometaphase arrest in zebrafish embryos, accompanied by congression failure and mitotic spindle abnormality. (A) A comparison of the percentage of cells in the indicated stages of mitosis, as judged by the degree of chromosome condensation, nuclear envelope breakdown (NEBD), and chromosome arrangement relative to spindle microtubules. (B) Embryos injected with 0.5 pmol of control or plk1 ATG MO were co-immunostained with anti-α-tubulin and anti-γ-tubulin antibodies at 24 hpf. Red, α-tubulin; green, γ-tubulin. Arrows mark uncongressed chromosomes. (C) Analyses of single cells from the embryos of Fig. 2H (and supplemental Fig. 2). Scale bar, 5 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 345(1), Jeong, K., Jeong, J.Y., Lee, H.O., Choi, E., and Lee, H., Inhibition of Plk1 induces mitotic infidelity and embryonic growth defects in developing zebrafish embryos, 34-48, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.