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Fig. S4 elys mRNA is maternally inherited, initially expressed ubiquitously, and later restricted to tissues with high levels of proliferation. (A) RT-PCR of the first two exons of elys and the entire open reading frame of both actin and claudin a. Numbers above each lane designate hpf. (B-G) elys expression in the eye. At 1 dpf, elys is expressed throughout the retina (B,C), but by 2 and 3 dpf its expression is restricted to the CMZ (D-G). Whole-mount dorsal views (B,D,F) and lateral views of dissected eyes (C,E,G). (H,I) elys is also strongly expressed in the developing intestine. (H) Lateral view, whole-mount; (I) frontal cross-section.