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Fig. S3 Signals required for neurogenesis in the early differentiating central retina are delayed and reduced in flo eyes. (A) Lateral views of dissected eyes revealing the stereotypical spatial and temporal expression pattern of ath5 (top) that presages the generation of the first-born retinal neurons: the RGCs. This pattern is delayed and reduced in flo mutants (bottom). (B) Lateral confocal projections of live wild-type and flo embryos expressing GFP from the shh promoter showing the spread of Shh signal throughout the central retina as neurogenesis proceeds.
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