ZFIN Image: Devireddy et al., 2010, Fig. 3

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Figure Caption

Fig. 3 BDH2 Is Required for 24p3-Mediated Iron Transport and Apoptosis

(A) Iron-binding assay. Left: FL5.12 cells were treated in the presence or absence of IL-3, and with a Bdh2 or control shRNA, and the iron-binding ability of 24p3 in the CM was monitored as described in Figure 1B. Inset: immunoblot analysis monitoring 24p3 levels in the CM and tubulin levels in the whole-cell extract (WCE). Right: FL5.12/Ec-24p3 cells were treated in the presence or absence of ponasterone A. Error bars indicate the SD.

(B) Iron exocytosis assay. ⁵⁵FeCl₃-loaded FL5.12 cells were either untreated (control) or treated with apo-24p3 or DFO, and the presence of ⁵⁵Fe in the medium was quantified. Error bars indicate the SD.

(C) Apoptosis assay. FL5.12 cells cultured in the presence or absence of IL-3, with or without 2,5-DHBA, and expressing a NS or Bdh2 shRNA were analyzed for apoptosis by annexin V-FITC staining. Error bars indicate the SD.

Acknowledgments

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Reprinted from Cell, 141(6), Devireddy, L.R., Hart, D.O., Goetz, D.H., and Green, M.R., A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production, 1006-1017, Copyright (2010) with permission from Elsevier. Full text @ Cell