ZFIN Image: Lindeman et al., 2010, Fig. 2

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Figure Caption

Fig. 2 Expression pattern of selected genes in zebrafish embryos. (A) Data extracted from Agilent microarray analyses of gene expression in unfertilized eggs and MBT embryos. Data was generated from 3 biological replicates for both stages, with 3 and 4 arrays for unfertilized egg and MBT stages, respectively. Signals were quantile-normalized, multiple probes for each gene were aggregated using the median, and mean±SD calculated based on log₂-transformed values. Negative controls are the mean of the replicates of the median of 150 different negative control probes scattered throughout the array. (B) RT-PCR analysis of expression of indicated genes in MBT⁺ embryos; -RT, PCR without reverse transcription.

Acknowledgments

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