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Fig. S6 Phenotypes of pcdh18a and pcdh18b knockdowns. Bright-field images of live embryos at 10–11.5 hpf and 31–31.5 hpf are shown. (A) Uninjected control (Ctr) embryos. (B–D) Single and double knockdowns of pcdh18a/18b. Moderate amounts of MO (3.6 ng) were used for single KD (B,C) and a 1:1 mixture of two MOs (3.6 ng each) was injected for double KD (D). Double knockdown embryos showed a shorter anteroposterior axis than the single knockdown embryos, indicative of more severe defects in C&E movements and suggesting a degree of functional redundancy between pcdh18a and pcdh18b.
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