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Fig. S4 Sec24c knockdown strategy. (A,B) Schematic representation of the knockdown strategy for sec24c and the location of the translation blocking morpholinos: UTR-MO (A), ATG-MO (B). (C) Schematic drawing of the construct containing 238 bp of sec24c mRNA fused in frame to three HA tags in the pCS2+ vector that was used to test the effectiveness of translation inhibition by the morpholinos. (D,E) Injection of 100 pg of the HA tagged construct into 1-cell stage embryos shows expression as visualized by HA tag recognizing antibody in red (D). Injection of 100 pg of the HA tagged construct in combination with 2.0 ng of MO effectively blocked translation of the reporter construct (E).