Analysis of locomotive movement by measuring the swimming distance in 30 seconds. The WD40 morphants swam much less distance than the wild-type fish. This locomotive defect could be rescued by over-expressing either zLRRK2 (WD40+zLRRK2) or hLRRK2 (WD40+hLRRK2) mRNA as well as by L-dopa treatment (WD40 + L-dopa). Each bar indicates the distance of an individual fish moved within 30 seconds. Each red dot (connected by red line) indicates the average distance of each of the five fish pools moved within 30 seconds, and the bar represents the SD of distance. *P<0.05, **P<0.01, n.s.: not significant (unpaired Student′s t-test). It is noticeable that one of the ΔWD40 fish showed a very different phenotype from the rest. The distinct phenotype of this ‘outlier’ fish was likely due to either the failure or in-efficient knock-down effect by morpholino. The rescue effect by over-expressing hLRRK2 became statistically significant (P = 0.03) when the possible outlier of the ΔWD40 group (black arrow) is removed from the statistical analysis.