ZFIN Image: Branam et al., 2010, Fig. S1

Image

Figure Caption

Fig. S1 Chd can rescue embryos ventralized by co-injection of Chl- and Chd-MOs. (A) Embryos were dorsalized by injection of RNA (80 pg) encoding Chd. Also shown, for comparison, is a representative embryo injected with 0.38 mM StCon-MO. Embryos co-injected with Chd-MO (.026 mM) and Chl-MO (0.1mM) are moderately to severely ventralized, whereas overexpression of Chd RNA (80 pg) rescues embryos co-injected with Chd-MO and Chl 5′1, such that many are only mildly ventralized or mildly (left bottom panel) or strongly dorsalized (right bottom panel). (B) Quantification is shown of phenotypes at 24 hpf. Co-injection with p53-MO was to control for the possibility of off-target effects.

Acknowledgments

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Reprinted from Developmental Biology, 341(2), Branam, A.M., Hoffman, G.G., Pelegri, F., and Greenspan, D.S., Zebrafish Chordin-like and Chordin Are Functionally Redundant in Regulating Patterning of the Dorsoventral Axis, 444-458, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.