|
Fig. 6 AGN192403 can permanently inhibit regeneration of some fin rays. (A) Fish were treated with AGN192403 for 7 days after fin amputation. Compound was then removed and regeneration was allowed to proceed for 30 days (25°C). Regeneration proceeded normally in some rays (arrow) but was completely absent in others (double arrow). (B) When these same fins were reamputated proximal to the original amputation plane, regeneration proceeded normally. (C) After fin amputation and 7 days of AGN192403 or DMSO treatment, fish were recovered for 2 weeks in fresh water (no drug) and the degree of fin regeneration was quantified for the longest (V3) and shortest (V9) fin rays. AGN192403-treated fish failed to completely restore their fins. These data were significant for both V3 and V9 (p < 0.05) (data represent means ± SEM).