ZFIN Image: Mao et al., 2009, Fig. 3

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Figure Caption

Fig. 3 Ectopic Activation of Shh and Shh Targets Results from an Inhibition of Etv-Dependent Regulation

(A) Shh expression at different stages of limb development in R26-EtvEnR and Prx1-Cre;R26-EtvEnR embryos.

(B) Expression of Gli1, Hand2, Alx4, and Gremlin transcripts in the limb buds of R26-EtvEnR and Prx1-Cre;R26-EtvEnR embryos at E10.5.

(C) Dorsal view of Shh expression in the zebrafish pectoral fin bud 48 hr postfertilization (upper); a higher-magnification view (lower). The scale bars represent 0.02 mm.

(D) Quantitative real-time PCR analysis of Gli1 mRNA levels after culturing of anterior limb bud cells for 24 hr in the presence or absence of Fgf8.

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Acknowledgments

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Reprinted from Developmental Cell, 16(4), Mao, J., McGlinn, E., Huang, P., Tabin, C.J., and McMahon, A.P., Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb, 600-606, Copyright (2009) with permission from Elsevier. Full text @ Dev. Cell