Fig. S2

Fig. S2

Pbx-depleted embryos exhibit defects in primitive hematopoietic gene expression. Shown are representative embryos following in situ hybridization analysis of hematopoietic marker expression in wild type (WT; A-G) compared with Pbx-depleted (A′-G′) embryos. (A-D′) The PLM of 16 hpf flat-mounted, deyolked embryos is shown in dorsal view with anterior to the left. gata1 expression is severely reduced in Pbx-depleted embryos (A′ 77%, n = 31) when compared to WT (A). flk1a expression is unchanged in Pbx-depleted embryos (B′ 100%, n = 31) when compared to WT (B). Lateral domain of scl (90%, n = 20) and lmo2 expression (90%, n = 31) is abolished in Pbx-depleted embryos (C′, D′ arrowheads), while the medial domain of scl and lmo2 expression is near normal. (E-G′) 24 hpf whole-mount embryos are shown in lateral view with anterior to the left. gata1 (E′ 88%, n = 66), scl (F′ 90%, n = 30), and lmo2 (G′ 94%, n = 32) expression is reduced in the ICM of Pbx-depleted embryos when compared to WT (E-G).