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Fig. 5 Overexpressing hoxb7a rescues gata1 erythroid gene expression in Pbx-depleted but not meis1-morphant embryos. (A–C) Shown are representative embryos following in situ hybridization analysis of gata1 expression in 16 hpf embryos. Dorsal view of PLM gene expression is shown in flat-mounted and deyolked embryos, with anterior oriented to the left. Both meis1-morphant embryos (B; 94%, n = 63) and hoxb7a RNA-injected meis1-morphant embryos (C; 91%, n = 43) exhibit a severe decrease in gata1 expression when compared to wild type embryos (WT; A). (D) Quantitative real-time PCR analysis of gata1 expression in 16 hpf wild type embryos (WT), meis1-morphant embryos (meis1 MO), and hoxb7a RNA-injected meis1-morphant embryos (meis1 MO; hoxb7a RNA). Shown is the relative quantity of gata1 expression. Samples were normalized to ef1a and WT was set to 1. Error bars indicate standard deviation from the mean. *Indicates the difference compared with WT is significant by Student t test; P < 0.0001.
Reprinted from Developmental Biology, 340(2), Pillay, L.M., Forrester, A.M., Erickson, T., Berman, J.N., and Waskiewicz, A.J., The Hox cofactors Meis1 and Pbx act upstream of gata1 to regulate primitive hematopoiesis, 306-317, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.