ZFIN Image: Punnamoottil et al., 2010, Fig. 10

Image

Figure Caption

Fig. 10 A. Three conserved sequences, identified through VISTA alignments of the zebrafish Hox4 loci (bf1, bf2, bf4). B. Alignments of the amphioxus intronic element with the four paralogous zebrafish intronic elements (upper part) and the frog intronic elements (lower part). The width of the line is proportional to the length of the local alignment, the color indicates the level of similarity. C. The intronic element of the AmphiHox4 gene, bf4, was tested in zebrafish transgenesis. Shown is are larvae with GFP expression in the pharyngeal arches (blue arrow) and the pectoral fins (pink arrows) as well as hindbrain (red arrow) and spinal cord (yellow arrow). D. For comparison the expression features that are regulated through the tested zebrafish intron elements are shown. The arrows use the following designated color code: red, hindbrain; yellow, spinal cord; blue, pharyngeal arches and pink, pectoral fins.

Acknowledgments

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Reprinted from Developmental Biology, 340(2), Punnamoottil, B., Herrmann, C., Pascual Anaya, J., D'Aniello, S., Garcia-Fern�ndez, J., Akalin, A., Becker, T.S., and Rinkwitz, S., Cis-regulatory characterization of sequence conservation surrounding the Hox4 genes, 269-282, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.